The adulticidal efficacy profile of the extracts, as measured by the percentage of the adult parasites killed at the end of observation period, is as follows: 100 and 95% at concentration of 10 mg/ml, 97.5 and 92.6% at concentration of 5 mg/ml, 95.08 and 91.4% at concentration of 2.5 mg/ml, and 91.4 and 89.4% at concentration of 1.25 mg/mL for the C. quadrangularis and S. molle, respectively.
To prepare different concentrations: 1- The extract is dissolved in the minimum amount of suitable solvent and then a quantitative transfer is done to a volumetric flask with a final size of 100 ml...
taxonomic authentification of plant material sample. Specification No. : NBRI-SOP-202, Receipt no. & date : 19/76, 27-2-2009 Preparation of the extracts Plant extracts were prepared using three different extracting solvents: A. Ether extract: The powdered plant material (20 gm of adhatoda vasica leaves) was extracted with 250 ml of petroleum
2. Phenolic Sample Preparation and Characterization 2.1. Extraction The extraction of bioactive compounds from plant materials is the first step in the utilization of phytochemicals in the preparation of dietary supplements or nutraceuticals, food ingredients, pharmaceutical, and cosmetic products.
To about 1 g of each plant extract in the test tube, 10 mL distilled water was added and the mixture boiled for 5 min. The mixture was filtered while hot and the cooled filtrate made alkaline to litmus paper with 20% sodium hydroxide solution.
The stock solution of the extract was prepared by dissolving 10 mg of plant extract in 10 ml DMSO. The following concentrations were prepared, 50, 25, 12.5, 6.25, 3.13, 1.56, and 0.78 μg/ml of the crude extract for determination of minimum inhibitory concentration, minimum bactericidal concentration (MBC), and minimum fungicidal concentration.
Apr 01, 2016· Preparation of plant extracts for screening and assay plate preparation. Plant extracts (Table 1) were prepared by the Sarawak Biodiversity Centre (SBC), Kuching, Malaysia, as described previously .Briefly, whole plants, or parts thereof, were dried, ground into a powder, extracted into 1:1 v/v dichloromethane:methanol and then concentrated using a rotary evaporator.
Dec 25, 2001· The aim of this study was to test a large number of essential oils and plant extracts against a diverse range of organisms comprising Gram‐positive and Gram‐negative bacteria and a yeast. The purpose of this was to create directly comparable, quantitative, antimicrobial data and to generate data for oils for which little data exist.
2.5. Test for Phenols and Tannins. Extract was mixed with 2 mL of 2% solution of FeCl 3. A blue-green or black coloration indicated the presence of phenols and tannins (Figure 2(b)). 2.6. Test for Terpenoids (Salkowski's Test) Extract was mixed with 2 mL of chloroform. Then 2 mL of concentrated sulfuric acid was added carefully and shaken gently.
Jul 10, 2013· Antibacterial activity of the plant extracts. The antibacterial activity of the plant extracts are depicted in Table 3.The results indicated that the plants extracts showed antibacterial activities at variable degrees against MDR bacteria, with MICs values varying from 32 to 1024 μg/ml. Extracts of A. digitata displayed the most important spectrum of activity, its inhibitory effects being ...
Apr 01, 2016· The MIC is the lowest concentration of antimicrobial agent that completely inhibits growth of the organism in tubes or microdilution wells as detected by the unaided eye .Unlike microdilution method, the main disadvantages of the macrodilution method are the tedious, manual undertaking, risk of errors in the preparation of antimicrobial solutions for each test, and the comparatively large ...
Sep 01, 2011· Different concentrations (5, 10, 15 and 20%) prepared from these extracts inhibited the growth of the test pathogens and the effect gradually increased with concentration. The 20% ethyl acetate extract gave the strongest inhibition compared with the activity obtained by the same concentration of the other extracts.
The test disc was prepared by incorporating 20 µL of each extract (50, 100 and 500 mg/ml) to 6 mm sterilized filter paper disc to give a final concentration of 1, 2 and 10 mg/disc. The discs were left to dry under Fairuz Fadhilah Mohd Jalaniet al: Asian Journal of .
minimal inhibitory concentration (MIC) values of plant extracts. Plant extracts were serially diluted, ranging from 1/2 up to a 1/100 dilution from the crude extract. In each well, 100 µL of each extract dilution was mixed with 100 Table 1. List of medicinal plants used in this work with the popular uses in Uruguay. Lonicera japonica Thunb.
2.3.6 Preparation of extract dilutions A stock solution of plant aqueous extract was prepared by dissolving 100 mg of extract in 1 ml of sterile distilled water. Likewise, for the ethanol extract, 100 mg of extract was dissolved in 1 ml of 10 % Tween-20 rather than the original solvent (i.e. Dimethyl sulfoxide), and so the initial concentration ...
Jan 23, 2014· To prepare the extract, 5 g each of powdered plant was extracted using soxhlet extraction unit using 70 ml of methanol as the solvent at 60°C and the extract was collected in the upper chamber. Filtered extract was concentrated to evaporate the methanol completely in vacuum centrifuge and the dried extract was stored in −20°C.
Chemical composition of plant extracts by chromatography-mass spectrometry (GC-MS) revealed five different compounds in F. vulgare: Piperidine, 3-isopropyl, Bicyclo[2.2.1]heptan-2-one, 1,3,3 ...
Preparation of plant ... separated in test tubes by centrifugation 3000 rpm, the filtrate was dried in oven 37 C for 24 hrs. The final product was stored in freezer at (-20) C (Krell et al., ... FIGURE 1: The inhibitory effect of different concentration of Garlic extract on the growth of Staphylococcus aureus.
Nov 18, 2016· The effectiveness of using Tilia cordata extract as a green corrosion inhibitor for carbon steel in 1 M hydrochloric acid solutions was demonstrated by employing some chemical and electrochemical techniques. The surface morphology of C-steel specimens was examined. The results showed that Tilia cordata has corrosion inhibition characteristics with efficiency of 96% as the concentration .
The termiticidal action of the aqueous seed extracts showed that mortality was achieved faster at higher concentrations than at lower concentration levels. At 0.4 g mL −1 treatment, all the termites died within 40 minutes while it took 60 minutes for all the termite workers to die when exposed to the 0.1 g mL −1 treatment (Figure 1 ).
In this test, 2 mL of acetic anhydride was added to 0.5 g of plant extract with 2 mL concentrated H 2 SO 4. The colour change from violet to blue or green is an indication of steroids. Test for Tannins. In the test for tannins, 0.5 g of plant extract was boiled in 20 mL of water in a test tube and filtered.
Many investigations of plant extracts have been per-formed with different coronaviruses. The main targets were proteins involved in coronaviral replication, prote-ases and ion channel conductance . Only a few in-vestigations have been performed to test the anti-IBV activity of plant extracts. Several studies have found that the plant ...
Mar 25, 2018· Plants are rich in secondary metabolites and are being used for the treatment of various ailments in the indigenous system of medicine. Many developing countries are facing illnesses, and deaths among children are caused by germs, which get into the mouth via water and food. In addition, it has been estimated that up to 80% of all disease and sickness in the world is caused by inadequate ...
May 27, 2015· Conditions of preparation of different propolis extracts Spectrophotometric evaluations Total content of phenolic compounds in extracts was determined spectrophotometrically using the Folin-Ciocalteu method [ 15 ]. 0.1 mL extract was mixed with 2.5 mL distilled water, 0.1 mL of Folin-Ciocalteu reagent and 0.5 mL of 20 % sodium carbonate.